Determination of lead and isotope ratios in whole blood by inductively coupled plasma mass spectrometry / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To establish a method to determine lead and Isotope Ratios in whole blood by inductively coupled plasma mass spectrometry (ICP-MS).</p><p><b>METHODS</b>The whole blood samples was removed protein by 5% nitric acid , Online join thallium (Tl) as internal standard substance, used lyophilized bovine blood lead and cadmium standard material (GBW09139h and GBW09140h) for quality control of blood lead concentrations. Lead isotope standard substances (GBW04426) is used to determine the correction factor, lead isotope ratios will lead isotope standard reference material NIST SRM981 by icp-ms with samples for testing.</p><p><b>RESULTS</b>Optimize the detection method, detection of blood lead and lead isotope, and the method of linear range r >0.9999, GBW09139h and GBW09140h test results are within the scope of quality control. NIST SRM981 isotope determination precision RSD<1%, NIST SRM981 test results and the certificate of value close to.</p><p><b>CONCLUSION</b>The method is simple and convenient data is reliable, can meet the total lead (pb) in blood and former isotope simultaneous determination.</p>

Determining lead sources in Mexico using the lead isotope ratio

OBJECTIVE: Lead poisoning can, in some cases, be traced to a specific route or source of exposure on the basis of the individual's blood lead isotope ratio. To assess the major source of lead exposure among women residing in Mexico City, we compared blood, ceramic, and gasoline lead isotope ratios. MATERIAL AND METHODS: The study population, randomly selected from participants of a large trial, (1/1996-12/1996) comprised of 16 women whose lead levels exceeded 10 æg/dl and who reported using lead-glazed ceramics. Lead isotope ratios were performed on a Perkin Elmer 5000 Inductively Coupled Plasma Mass Spectrometer (ICP-MS) interfaced with a Perkin Elmer HGA-600MS Electrothermal Vaporization System (ETV). RESULTS: The isotope ratios (206Pb/204Pb, 207Pb/204Pb, and 208Pb/204Pb) of both the blood specimens and their corresponding ceramic specimens were highly correlated, with r=0.9979, r²=0.9958, r=0.9957, r²=0.9915 and r=0.9945, r²=0.9890 values for the three isotope ratios, respectively, suggesting that the lead exposure most likely resulted from the use of these ceramic. Measurements of lead isotope ratios from leaded gasoline in use at the time of blood sampling, differed from those in blood and ceramics. CONCLUSIONS: Determining lead isotope ratios can be an efficient tool to identify a major source of lead exposure and to support the implementation of public health prevention and control measures.

Correlations between ventilatory function tests, arterid blood gases and blood levels of some radioactive isotopes in asymptomatic and symptomatic cigarette smokers

In this work, three groups of subjects were studied. The first was control group [8 cases], the second was asymptomatic cigarette smokers [30 cases] and the third was symptomatic cigarette smokers [36 cases] that was in turn divided into three equal subgroups: asthma, COPD and cancer lung. All groups were examined for ventilatory function tests [FVC, FEV1 -FEV1% and FEF 25-75%], arterial blood gases, [Pa O2 and PaCO2, pH] and blood levels of lead 212, lead214, and Cesium 137. There was a significant decrease in all ventilalory function tests, PaO 2and increase PaCO2 in symptomatic group in relation to control and asymptomatic groups. The pH of blood showed no change. The study showed a significant increase in the levels of lead 212, lead214 and cesium137 in both smokers groups in relation to control. FEV1 and FEF 25-75%, lead212, lead 214 and cesium 137 showed significant differences between the three studied groups. Also there was a significant negative correlation between the blood lead 212 and the FEV1% in the symptomatic cigarette smokers group. These changes were related to the toxic contents of cigarette leaves and wrappings including the high content of the previously estimated radioisotopes